The effects of Biological Self Restore as an immune system booster on nonspecific resistance of the organism was studied under experimental conditions on outbred white fattening rats weighing 35-40 g infected with radionuclides during their development to a mature state.
Animals were divided into three groups: group 1 received vivarium feed, group 2 received special food , which was issued daily at the rate of 1 g per animal for 3 months.
Group 3, in addition to special food, received BSR in a daily amount from 1.5 to 3.0 mg per animal, during the experiment it was found that in animals treated with special food and this product, there were no significant violations in the leukograms, whereas animals receiving only special food, lymphopenia was recorded, in connection with this it was necessary to determine the functional activity of lymphocytes before and after the introduction of this product.
It is known that during intoxications, including radioactive ones, the structure of the nuclear apparatus of peripheral blood lymphocytes undergoes changes, the microstructure of the nucleus was studied using the method of cytochemical staining of RNA in smears of peripheral blood with 0.125% methylene blue solution.
From the table. Figure 5 shows that the nucleolar apparatus of lymphocytes in animals treated with radioactive food was significantly different from animals in the intact group. So, with a significant decrease in the number of lymphocytes with compact nucleols, an increase in the number of lymphocytes with micronucleols was observed. The introduction of BSR simultaneously with the radioactive feed led to a significant increase in the number of lymphocytes with compact nucleols and a decrease in their number with micronucleols, since the presence of compact nucleols indicates a state of active RNA synthesis, and an increase in the number of lymphocytes with micronucleols indicates a state of irreversible degeneration and decay, then a legitimate conclusion was made that BSR delays the development of metabolic and structural disorders in cells.
Table 5
Indicators of the functional state of lymphocytes in animals treated radioactive feed and BSR
For a comparative study of the effect of BSR on indicators characterizing the body’s defenses, the generally accepted test of the phagocytic activity of peripheral blood neutrophils was used, which reflects the state of nonspecific reactivity of the body.
Presented in the table. 6, the results of the study indicate a significant increase in the attraction ability of neutrophils in groups of animals treated with the tested drugs, compared with control animals fed with food contaminated with radionuclides. In practice, the use of biological products in the blood restored the number of neutrophils capable of adsorbing microorganisms. The phagocytic numbers in the experimental and control groups of the animals studied did not differ much, indicating that the number of neutrophils remaining in all groups was the same, but phagocytosis was significantly higher in the group of animals treated with BSR, which, thus, contributed to the preservation of a significantly larger the number of active neutrophils capable of phagocytosis.
Table 6
Phagocytic activity of peripheral blood neutrophils in animals treated with BSR for 2 months
* preparations were introduced into the feed at the rate of 1.5 mg per 100 g of live weight of experimental animals
To assess the digestive activity of neutrophils, that is, the second stage of phagocytosis, the ratio of the number of killed bacteria to the total number of phagocytized microbes was analyzed. It was found that in the group of animals treated with BSR, the digestion and digestion index was 2 times higher than in animals of the control group.
In clinical conditions, the effect of BSR on cellular immunity as an immune system booster was studied on the basis of a comprehensive examination of 118 people exposed to increased radiation. As a result of studying the dynamics of the indicator of TB of the lymphocyte system, it was found that in all examined before taking BSR a decrease in the absolute number of lymphocytes was observed (2078 211 in 1 μl instead of 2395 355).
A decrease in their percentage was also statistically significant (19.3 1.7% instead of 44.7 0.8% in the control).
Thus, a sharp violation of the ratios between immunocompetent blood cells was found in the examined individuals: the T / B coefficient was 1.1 instead of 3.3 in the control, which indicated a pronounced inhibition of the body’s immune system.
Reception of BSR for 26 days contributed to a significant decrease in the content of O-lymphocytes from 64.0 2.0% to 49.0 1.3% and a tendency to normalize the content of T and B lymphocytes from 435.0 44.7 to 690, 0 102.0 in 1 μl and from 364.0 4.7 to 290.0 62.8 in 1 μl, respectively.
Thus, the T / B coefficient approached the norm, which indicated the normalization of immunocompetent cell ratios in the blood.
Considering that in the examined population, as in animal experiments, when taking BSR an increase in the phagocytic activity of blood neutrophils was recorded, it was necessary to find out the level of restoration of the metabolic activity of phagocytic cells. The functional reserve of these cells was determined by determining the difference between the stimulated (with the addition of inert latex particles) and the spontaneous HCT test, the so-called HCT recovery test (nitrosine tetrazole).
As can be seen from the table. 7, the degree of stimulation was significantly lower in the examined patients than in healthy individuals. The use of BSR helped restore the spontaneous HCT test. However, significant differences in the effectiveness of the use of these drugs were revealed when studying the functional reserve of phagocytic cells. Reception of BSR allowed to restore the reserve capacity of these cells by 1.8 times.
Table 7
Changes in the parameters of the NBT test and functional reserve of phagocytic cells under the influence of BSR and (prototype)
Thus, laboratory and clinical trials confirmed the higher physiological activity of BSR compared to other fungus lines, expressed in its greater efficiency in restoring the latent reserve activity of oxygen-dependent metabolism of phagocytic cells.
The positive effect of BSR on the restoration of nonspecific immunity of the body was also confirmed by the results of the inclusion of BSR in comparison with the prototype in the standard treatment regimen for patients suffering from gastrointestinal diseases (gastroduodenitis).
Observations were carried out for three groups of patients. A control group of 30 patients received only conventional treatment, and BSR, respectively, were included in the treatment regimen for patients of two experimental groups of 26 people each. Klemparsky test results, reflected in the table. 6, testified to pronounced violations of the body’s defences in all patients.
The inclusion of both biological products in conventional treatment significantly helped the body restore the bactericidal properties of the skin. This is evidenced by a significant decrease in seeding on the prints of the skin of patients of the experimental groups, not only of the total number of microorganisms, but also a change in their qualitative composition: a decrease in the number of hemolytic and mannitol-degrading forms. Moreover, as can be seen from the data table. 8, the effectiveness of BSR.
Table 8
Indicators of skin microflora in persons treated for diseases of the gastrointestinal tract, in recalculation on 1 sq cm of skin surface (test Klemparskaya)
The data given in table. 9, indicate the positive effect of BSR and with violations of the intestinal biocenosis.
Table 9
Indicators of fecal microflora of patients (per 1 g)
In the table. 10 shows the results of studying the dynamics of the enzyme lysozyme in groups of patients with gastrointestinal diseases when taking BSR, and in the control group of patients who received conventional treatment.
As is known, the enzyme lysozyme is produced by leukocytes, has bactericidal activity and, contained in many biological media, provides a barrier function from the penetration of pathogenic microorganisms into the body, preventing the generalization and chronicity of inflammatory processes, as well as the development of dysbacterioses.
From the data table. 10 shows that in patients of all three groups, lysozyme in saliva, blood and stomach contents was reduced. The use of BSR significantly contributed to the partial restoration of lysozyme activity in saliva and gastric juice. The data obtained allow us to recommend BSR for inclusion in conventional treatment in patients with reduced protective properties of the mucous membranes of the oral cavity and gastrointestinal canal.
Table 10
Dynamics of lysozyme content in different biological environments of patients with diseases of the gastrointestinal tract (in a conditional unit by the Dorofeychuk method)
Thus, an extensive set of studies revealed a higher efficiency of BSR as a stimulator of nonspecific immunity of an organism (immune system booster).
The effectiveness of the use of BSR for disorders of the immune system was also studied against the background of the development of autoimmune processes in the body.
Autoimmune processes are caused by antigens of the body’s own tissues and are accompanied by the appearance of antibodies or sensitized lymphocytes in situations where the body loses its tolerance to its own tissue antigens and begins to reject its own tissues, taking them for foreign ones. According to the accepted scientific theory, autoimmunization is the result of the destabilization of cell membranes of tissues and organs that occurs under the influence of various damaging factors, and is a pathological process.
The effect of BSR on autoimmune processes was studied with its oral administration at a dose of 5 g per day for 14 days in 47 people exposed to high levels of radiation. The studies were carried out according to the original method, in which the registration of autoimmune reactions was carried out by increasing the immunochemiluminescent glow in a sample containing a specific autoimmunization antigen, compared with a sample without antigen.
Before and after the use of BSR, autoantibodies to organ-specific antigens of the heart, liver, lung, pancreas, stomach, kidneys, small and large intestines, and also the thyroid gland were determined. Additional studies have established that electrophoretic mobility of these autoantibodies are beta and gamma globulins.
As the results of the study showed, in all individuals exposed to radiation, an intensification of the production of autoantibodies was observed.
As can be seen from the table. 11, positive reactions to the presence of anti-tissue autoantibodies to all antigens used in the studies were observed in 15 people (group 1 of the examined). Moreover, clinically pronounced manifestations of the disease, mainly in the form of vegetative-vascular dystonia of the hypertonic type, were detected only in 9 of them. These were persons aged 41-60 years. In 6 people under the age of 40 years, an increase in the level of anti-tissue antibodies was not accompanied by clinically confirmed diseases. The level of increase in autoantibodies in healthy individuals was 2-4 times higher than normal, while in clinically confirmed diseases, autoimmunization rates exceeded the norm by 5 or more times.
Table 11
Changes in autoimmunization rates by patients from group 1 who received BSR (n=15)
After the oral course of BSR was taken, the intensity of autoimmunization changed, however, the severity of these changes for different antigens was not the same, a significant decrease in the concentration of autoantibodies in group 1 of the examined was observed only in relation to liver tissues (almost 2 times), kidneys (more than 3 times) and the large intestine (2.5 times), clinically, all individuals in this group showed an improvement in their general condition and a decrease in the frequency of blood pressure drops.
In the remaining 32 patients, an initial increase in the concentration of autoantibodies was detected only in relation to one or more antigens, however, an increase in the concentration of antibodies to these antigens was more pronounced than in group 1. These 32 people were divided into 2 groups: group 2 14 people and group 3 18 person.
In individuals of group 2, a pronounced increase in autoantibodies was detected only in relation to lung tissue (on average 9 or more times) and liver (8 or more times compared to normal). Clinically, these individuals showed pronounced signs of bronchitis (in the majority of smokers examined bronchitis) and chronic cholecystitis, the data are given in table. 12.
Table 12
Changes in autoimmunization rates by patients from group 2 who received BSR (n=14)
After the course of taking BSR, a significant decrease in autoimmunization processes was detected only in relation to liver tissue (2 or more times) and was not observed in relation to lung tissue. At the same time, a decrease in the clinical signs of cholecystitis was observed: a decrease in signs of intoxication, abdominal pain, and dyspeptic phenomena.
In 18 people of group 3 of the examined patients, a significant increase (7 or more times compared with the norm) of anti-tissue antibodies was observed in relation to the majority of the main parinchymal organs (kidneys, liver, pancreas), as well as the organs of the gastrointestinal tract (small and large intestines, stomach). All these individuals showed clinically expressed manifestations of diseases of the gastrointestinal tract in the form of chronic gastroduodenitis, chronic pancreatogenic colitis, chronic cholecystopancreatitis, chronic pancreatogenic colitis. Toxic-allergic kidney damage in these individuals was manifested in the form of signs of pyelocystitis and glomerulonephritis.
In this group, 10 patients took BSR . After drug administration, the most pronounced decrease in the level of anti-tissue antibodies was observed with respect to the kidney antigen (2–3.5 times), liver antigen (1.5–2 times), pancreas and large intestine (1.5–1.7 times) . As can be seen from the data given in table. 13.
Table 13
Changes in autoimmunization rates by patients from group 3 who received BSR and (prototype) (n=18)
All examined patients showed a significant increase in the production of antibodies to the thyroid gland, which is apparently due to the direct effect of radiation on this organ, as a result of the penetration of thyroid autoantigens into the blood, lymphocytes are sensitized to them and autoantibodies are produced. Autoallergic processes make it difficult to repair the thyroid gland.
In the course of biochemical studies, it was found that, unlike the prototype, the use of the BSR preparation reduced the autoimmune processes in relation to the thyroid gland, however, it was expressed moderately (1.5 times compared to the initial level) and was observed only in 67% of patients taking BSR.
The physiological effect of the drug lies in its ability to influence tissue metabolism and effectively affect the body’s immune systems, helping to increase non-specific immunity and suppress autoimmune processes. According to the effects obtained, the drug can be used in schemes for the prevention and treatment of somatic diseases in people living or working in environmentally hazardous conditions, including increased radiation; for the prevention and treatment of dysbiosis; as an additional tool in the complex treatment of cardiovascular diseases and diseases of the gastrointestinal tract; for the prevention and treatment of disorders of the hematopoietic function and with a shift in biochemical blood parameters, with a decrease in the body’s resistance (immunosuppression).
As you can see Biological Self Restore has an immune system booster when needed and immunomodulating as required by the host if the immune system is already over active.
