The effect of the product BSR on tissue metabolism.

The study of the effect of  on tissue metabolism was carried out under clinical conditions in groups of patients from high radiation areas. In this case, the effect of the drug on lipid metabolism and on lipid peroxidation was evaluated in patients with pathology of the digestive system and diseases of the cardiovascular system.

As is known, lipid metabolism disorders, expressed in hypercholesterolemia, are one of the main factors in the development of atherosclerosis, therefore, for clinical observations groups of overweight patients were combined with an increased content of cholesterol and triglycerides in the blood serum. One group of patients received traditional treatment, the other in addition to this treatment received 1 g of this product 2 times a day for 28 days. The blood levels of cholesterol, triglycerides, high density lipoproteins and sugar were analyzed as indicators of lipid metabolism. The dynamics of these indicators, as well as data on the weight loss of patients as a result of the treatment, are given in table 1.

TABLE 1. Clinical and biological parameters of overweight patients with combined hypercholesterolemia indicators.

*TAKING BSR 1 GRAM TWICE DAILY FOR 28 DAYS.

х Reception BSR 1 g 2 times a day for 28 days

 

A comparison of the treatment results of patients from the experimental and control groups shows that taking this product contributes to a more pronounced statistically significant decrease in the studied clinical and biochemical parameters. At the same time, this product has not only a hypocholesterolemic effect, but also contributes to the normalization of blood sugar, which indicates the feasibility of its use in the initial forms of diabetes.

One of the leading mechanisms of tissue metabolism disorders due to damage to cell membranes, in particular as a result of radioactive damage, is the development of processes of free radical lipid oxidation, the suppression of which requires the entry into the body of compounds with antioxidant activity.

These compounds block the lipid peroxidation of plasma and subcellular membranes, protecting them from the action of peroxides and free radicals that are formed during radioactive damage, especially often in metabolically active cells.

In connection with the discovery of the lipotropic properties of this product , work was carried out to find out its effect on lipid peroxidation in the human body,

116 people from high radiation exposure groups were subjected to a comprehensive examination. Among the examined were mainly middle-aged people from 24 to 55 years. These patients were divided into two groups. Group 1 included patients with a predominant pathology of the digestive system (chronic gastroduodenitis, cholecystoangeocholitis, spastic atopic colitis).

Group 2 consisted of patients with predominant diseases of the cardiovascular system (hypertension, myocardial dystrophy, endoteritis, thrombophlebitis).

Patients of both groups received this product at 2-6 g per day for 28 days. Before and after the course of treatment, comprehensive clinical and instrumental, immuno-microbiological, biochemical and other studies were carried out.

During treatment, there were no cases of refusal of this product, no cases were found with an aggravation of the clinical course of the disease. On the 10-12th day of taking this product, 72.1% of the examined showed an improvement in their general state of health and a decrease in the intensity of earlier complaints. Objectively, more than 2/3 of the examined showed a positive dynamics of the main studied indicators (table. 2,3).

Free-radical processes were studied by the bio-chemiluminescent method, based on the super-weak glow of biological media and cells in the visible region of the spectrum, due to the presence of lipid structures, the study of ultra-weak glow was carried out using a Beta-2 analyzer, in which the PMT-39 is used as a photon counting detector. At the same time, spontaneous chemiluminescence indices characterize the level of free radicals, a fast flash of peroxide chemiluminescence, the level of lipid hydroperoxides, a slow flash of peroxide chemiluminescence, the level of organic peroxide radicals, in the presence of chemiluminescence inhibitors, decreases, as the concentration of free radicals decreases. Given the possibility of migration of peroxide radicals from the extracellular space into the cells,

As can be seen from the table. 2 and 3, in the subjects in the initial state, significant accumulations of products of free radical oxidation were determined in the blood. Reception of this product for 28 days contributed to a significant decrease in the intracellular content of peroxides, including organic peroxide radicals. This testified to the stabilizing effect of the drug on cell membranes and cell metabolism.

Table 2

Changes in indicators of free radical oxidation in the blood in diseases of the digestive system as a result of BSR administration.

Indicators	Blood plasma I                        II		Erythrocytes I                        II		Leukocytes I                        II
Spontaneous chemiluminescence Norm of indicator	92.3 +-3.3 80.5+-4.2	79.4+-5.6 80.5+-4.2	68.7+-2.4 34.6+-3.3	36.2+-4.1 34.6+-3.3	99.3+-3.2 39.6+-2.1	41.4+-4.3 39.6+-2.1
Peroxide chemiluminescence (fast flash) Norm of indicator	115.6+-9.8 294+-2.9	284+-12.3 294+-2.9	7512+-302 3260.7+-409.3	4234+-302 3260.7+-409.3	199.5+-2.7 84.2+-7.6	82.7+-7.6 84.2+-7.6
Peroxide chemiluminescence (slow flash) Norm of indicator	38.5+-2.9 64.7+-7.8	40.1+-7.6 64.7+-7.8	54.3+-7.9 31.8+-1.75	29.3+-3.1 31.8+-1.75	49.2+-4.4 13.8+-1.8	15.1+-4.2 13.8+-1.8

Note:   I – indicators before administration of the BSR,

II- indicators at the end of the administration of the BSR.

 

 

 

 

Table 3

Changes in indicators of free radical oxidation in the blood in diseases of the cardiovascular system as a result of BSR administration.

Indicators	Blood plasma I                        II		Erythrocytes I                        II		Leukocytes I                        II
Spontaneous chemiluminescence Norm of indicator	95.3+-5.2 80.5+-4.2	65.7+-2.1 80.5+-4.2	63.3+-1.4 34.6+-3.3	38.7+-3.2 34.6+-3.3	93.5+-70 39.6+-2.1	39.4+-1.5 39.6+-7.6
Peroxide chemiluminescence (fast flash) Norm of indicator	765+-35.4 294.9+-17.1	625.4+-39.2 294.9+-17.1	9542.6+-1005 3260.7+-409.3	4005+-205 3260.7+-409.3	150.67+-107 84.2+-7.6	87.23+-6.24
Peroxide chemiluminescence (slow flash) Norm of indicator	145.5+-17.1 64.7+-7.8	138.4+-20.1 64.7+-7.8	76.8+-1.5 31.8+-1.75	29.4+-2.2 31.8+-1.75	27.8+-3.2 13.8+-1.8	13.4+-2.4 13.8+-1.8

Note:   I – indicators before administration of BSR

II- indicators at the end of BSR administration.

Thus, the above results showed that BSR is able to stimulate the activity of the antioxidant systems of the body.

Experimental studies of the ability of this product to bind and excrete radionuclides that dramatically disrupt tissue metabolism in the body due to inactivation of enzyme proteins, were carried out on white rat pups weighing 35–40 g. In the experiment, the accumulation of radionuclides in muscle tissue was observed 4 groups of animals: control group 1, received vivarium feed: group 2 – received, along with ordinary food, special feeds at the rate of 1 g per animal per day (wheat with a total activity of Cs-134 and Cs-137 12.9  10 -8 curie / kg and cottage cheese 40.7  10 -8curie / kg); Groups 3 and 4 received, along with special food and this product, respectively, at the rate of 1.5 to 3 mg per animal. Feed was given daily for 3 months.

From the results presented in table. 4, it can be seen that the simultaneous administration of radioactive food and test drugs into the body prevented the accumulation of radioactive substances in the muscle tissue of animals, this product excreted radionuclides more actively, reducing their total content in the muscle tissue of animals by 8 times.

Table 4

The effect of BSR on the accumulation of Cs-134 and Cs-137 in rat muscle tissue

Animal groups	Total activity of radionuclides in muscle tissue, сurie /kg
Group 1 – intact (control) Group 2 – special feed Group 3 – special feed + BSR Group 4 – special feed + BSR (prototype)	10.3 x 10-10 88.9 x 10-8 11.2 x 10-8 40.2 x 10-8

This effect is directly related to the different content of polysaccharide fractions in the tested preparations, which play the role of a kind of biologically active factor that exerts a protective effect on cell membranes, affecting the magnitude of their surface charge and supporting static stress and metabolic activity in them.